John Lupton was born 1725 in Bucks County, Pennsylvania. His parents were Joseph Lupton and Mercy Twining. Some of John's descendants were known as the Round Hill or Presbyterian branch of the family.

John moved to Frederick County, Virginia with his family about 1740.

He married Sarah Frost at the Hopewell Monthly Meeting in Frederick County, Virginia, June 6, 1755. Sarah was the daughter of John and Mary Frost.

John Lupton, son of Joseph Lupton of Opeckan in the County of Frederick and Colony of Virginia, and Sarah Frost, daughter of John Frost of the County afforesaid; 26th day of 6th month, 1755; at Opeckan [Hopewell Meeting].

Sarah and John's children included:
Grace Lupton Steer (1757, married Joseph Steer),
Joshua Lupton (1759, married Lydia Reece/Rees - his stepsister),
Nathan Lupton (1761, married Margaret Reece/Rees -his stepsister),
Mary Lupton Raley (1764, married Eli Raley),
John Lupton (1769, married Elizabeth Cartmell),
Joseph Lupton (1771) and
Sarah Lupton Russell (1773).

In 1750, William signed his brother, Joseph's, marriage certificate at Hopewell in Frederick County, Virginia.

In November, 1770 John Branson named John Lupton as one of the executors of his will. John "refused to take upon himself the burthen of the Execution."

In 1771 the Hopewell Monthly Meeting in Frederick County, Virginia appointed John Lupton as one of those to meet the committee from the quarterly meeting.

In 1773, John signed Ann Lupton's marriage certificate.

Sarah died on May 22, 1775 when she was 40 years old.

After the death of his first wife, John married Ann Neill Reece/Rees on June 13, 1776 at the Hopewell Meeting. Ann was the daughter of Lewis Neill and Lydia Hollingsworth and the widow of Henry Reece/Rees She had at least five children already.

John and Ann's children included:
Elizabeth Lupton (1780), and
Jonah Lupton (1781).

The family remained in Frederick County during the American Revolution. It 1778 John pledged 5£ for

...the Benefit of the Indians, who were formerly the Native Owners of the Lands, on which we now live, or thire descendents if to be found, and if not for the service, & benefit of Other Indians....

John Lupton appeared in the 1782 census of Frederick County in the list of Robert White. Samuel Pickering was the next householder. There were 13 members of the household.

John died on December 25, 1804 in Frederick County. He left his large estate to his wife Ann, and his children, Joshua, Nathan, Grace Steer, John, Jr., Joseph, Mary Baley, Sarah Russell, and Elizabeth Lupton.

Ann died on October 27, 1826 in Goshen Auglaize County, Ohio.

Round Hill is an unincorporated community in Frederick County, Virginia just west of Winchester.